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[求助]
求助大神关于电化学测定小牛胸腺DNA
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各位大神： 小弟想求助一下关于电化学测定小牛胸腺DNA中的问题。 在文献中用加标的方法测定，他们都是得到鸟嘌呤和腺嘌呤的摩尔百分比（一般为the contents of adenine and guanine in thermally denatured DNA were calculated as 22.4% and 27.6% (in the molar ratio, mol%)请问这个怎么得出来的啊？ 我是称取一定量的小牛胸腺DNA然后热变性，但是怎么知道变性处理稀释后的DNA的摩尔值呢，如果不知道总的摩尔值如何能算出后面的摩尔百分比？ The determination of guanine and adenine concentrations was performed by standard addition method as follows. 50uL thermally denatured DNA solution was added in a beaker containing 10mL of pH 5.0 B–R buffer solution and the peak currents of guanine and adenine residue were measured firstly. Then a certain quantity standard solution of guanine and adenine was added and the peak currents of guanine and adenine were measured again. From the difference between the peak currents, the contents of adenine and guanine in thermally denatured DNA were calculated as 22.4% and 27.6% (in the molar ratio, mol%), respectively. Using the proposed method, the value (G + C)/(A + T) of 0.81 was obtained for thermally denatured DNA sample, which coincided to standard value of 0.77 |
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