| 查看: 1178 | 回复: 0 | ||
wovs211木虫 (正式写手)
|
[求助]
有关植物逆境胁迫下ASA-GSH关键代谢酶的测定
|
|
各位虫友，关于ASA-GSH代谢关键酶MDHAR的测定方法，我用的是下面的方法，饭是我测的黑麦草根系中这个MDHAR的OD值没有变化，不知道是怎么回事，十分着急，望虫友们提供帮助。 我的参考文献是Exogenous sodium nitroprusside and glutathione alleviate copper toxicity by reducing copper uptake and oxidative damage in rice (Oryza sativa L.) seedlings，Protoplasma (2014) 251:1373–1386。酶的提取液方法是To extract enzymes, fresh leaf samples (0.5 g) were homogenized separately with a reaction mixture containing 50 mM Kphosphate buffer (pH 7.0), 100 mM KCl, 1 mM AsA, 5 mMβ-mercaptoethanol, and 10 % (w/v) glycerol in pre-chilled mortars and pestles. The homogenate was centrifuged at12,500×g for 15 min and the resultant supernatants werecollected for analysis of enzyme activities and protein content.All procedures were performed at 0–4°C. 我提取的酶液定容到3ml.MDHAR (EC 1.6.5.4) activity was determined according to the method of Hossain et al. (1984). The reaction mixture contained 50 mM Tris–HCl buffer (pH 7.5), 0.2 mM NADPH, 2.5 mM AsA, 1.0 U of AO and enzyme solution in a final volume of 0.7 ml. The activity was calculated from the change in ascorbate at 340 nm for 1 min using the extinction coefficient of 6.2 mM−1 cm−1. 各位虫友，我测出OD值没有变化啊，怎么办啊。望虫友们提供帮组，谢谢。 |
回复此楼» 猜你喜欢
有多少人是今天查系统知道结果的？
已经有16人回复
-
我不理解！
已经有22人回复
-
2026年的国家社科基金项目通讯评审的新规则与新动向、新挑战
已经有10人回复
-
国自然面上复盘~欢迎讨论
已经有4人回复
-
系统查不到
已经有10人回复
-
基金不中，共勉
已经有3人回复
-
怎么查啊
已经有5人回复
-
范进中举一文的中心思想
已经有10人回复
-
2026国自然函评费到账
已经有25人回复
-
2026年8月25日国自然放榜前突然收到列入评审专家邮件，有关系吗？
已经有28人回复
