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Notoginsenosides, the main active gradients of Chinese traditional medicine Panax notoginseng, possessesa variety of biological activities including antioxidant property, anti-hyperglycemic, anti-obese,etc. However, pharmacokinetic evaluation for notoginsenosides is still a formidable task due to their lowconcentrations and complex components in vivo. The summation of this work generated a rapid and sensitivemethod for quantitative analysis of multi-notoginsenoside in rat plasma based on ultra fast liquidchromatographic-tandem mass spectrometric. After liquid–liquid extraction by n-butanol, notoginsenosideR1, Rg3, Rd, Rg2, Rb2, Rf, Rg1, Rb1 and Re were simultaneously monitored in negative ionizationmodeafter separating on a Thermo ODS C18 column (5mm 50mm×2.1mm) by a binary gradient elution, andall compounds were analyzed within 9 min. Multiple reaction monitoring (MRM) was performed as follows:R1 (m/z 967.7→637.4), Rg3 (m/z 819.6→621.4), Rd (m/z 981.6→783.5), Rg2 (m/z 819.6→475.4),Rb2 (m/z 1113.4→783.4), Rf (m/z 835.6→475.4), Rg1 (m/z 835.6→637.6), Rb1 (m/z 1143.7→945.6),Re (m/z 981.6→637.4), internal standard (digoxin, m/z 815.5→779.4). Validation parameters (linearity,sensitivity, intra-and inter-assay precision and accuracy, recovery and matrix effect) were withinacceptable ranges and biological extracts were stable during the entire storing and preparing process.This UFLC–MS/MS approach was further validated by being applied to the pharmacokinetic study for P.Notoginseng extract in rats, and the pharmacokinetic parameters were calculated by Winolin software.Thus, the presently developed methodology was simple, robust, accurate, precise, and would be usefulfor the pharmacokinetic studies for all kinds of notoginsenosides and other herbal saponins. |
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