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Gene Biotechnology,Second Edition
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Chapter 1 Strategies for Novel Research Projects Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries Chapter 4 Subcloning of Genes or DNA Fragments Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies Chapter 10 Analysis of Gene Expression at the Functional Genomic Level Chapter 11 Analysis of Gene Expression at the Proteomic Level Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH) Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH) In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression Chapter 17 New Strategies for Gene Knockout Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR Chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology — DNA Microarrays (Gene Chips) Chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology — Phage Display Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology |
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2015-12-21 22:05:05, 9.37 M
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