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[资源] Gene Biotechnology,Second Edition

Chapter 1
Strategies for Novel Research Projects
Chapter 2
Rapid Isolation of Specific cDNAs or Genes by PCR
Chapter 3
Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Chapter 4
Subcloning of Genes or DNA Fragments
Chapter 5
Nonisotopic and Isotopic DNA or RNA Sequencing
Chapter 6
Information Superhighway and Computer Databases of Nucleic Acids and Proteins
Chapter 7
Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8
Gene Overexpression by Sense RNA in Mammalian Systems
Chapter 9
Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
Chapter 10
Analysis of Gene Expression at the Functional Genomic Level
Chapter 11
Analysis of Gene Expression at the Proteomic Level
Chapter 12
Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH)
Chapter 13
Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH) In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs
Chapter 15
Isolation and Characterization of Genes from Genomic DNA Libraries
Chapter 16
Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression
Chapter 17
New Strategies for Gene Knockout
Chapter 18
Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
Chapter 19
Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR
Chapter 20
High-Throughput Analysis of Gene Expression by Cutting-Edge Technology — DNA Microarrays (Gene Chips)
Chapter 21
Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology — Phage Display
Chapter 22
Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology
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