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feiwanclamp银虫 (小有名气)
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[求助]
纳米簇的分离
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文献是这样说的：PAGE was performed with a Biorad Protean II XI system with gels of 3 mm thickness. The experimental conditions for PAGE are close to those employed in previous works.3 The total content of the acrylamide monomers were 3% (acrylamide/Bis, 94 : 6) and 25% (acrylamide/Bis, 93 : 7) for the stacking gel and separation gel, respectively. The staking and the separating gels were buffered at pH = 6.8 and 8.8, respectively, with Tris-HCl solution. The eluting buffer consisted of a solution of glycine (192 mM) and Tris (25 mM) in 80 : 20 (v/v) water : methanol. The purified NPs were dissolved in a 5% glycerol solution in water to a concentration of 4 mg.ml-1. The NPs solutions (2 ml) were loaded on a 3 mm gel without lanes and eluted for 17 hours at a constant voltage of 150 V to achieve separation. Parts of the gel containing each separated fraction were cut out and placed in Milli-Q water overnight. The gel lumps suspended in the solution were removed by filtration. NPs were finally further purified by dialysis in the same way as described above. For each ligand exchange reaction described below, 6 to 8 gels are necessary in order to get sufficient quantities of size separated NPs.（原文：J. AM. CHEM. SOC. 2008, 130, 7077–7084 9 7077） 就是选用native-page 将不同粒径的纳米簇分开，然后再将胶里面的纳米簇溶解； 我做到分离纳米簇以后想要将纳米簇提取出来，切碎以后，用水溶解，发现提取不出来，是胶配的太大还是有其他操作步骤没有注意到。请问有做过这一方面的专家么，能否给予小弟帮助，谢谢。@nowitzki_ci |
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