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小云云。新虫 (小有名气)
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[求助]
求大神帮忙把下面两段的文献摘要翻译成中文。。。
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1 A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice. 2 The coordination bond between gold and sulfur (Au–S) has been widely studied and utilized in many fields. However, detailed investigations on the basic nature of this bond are still lacking. A gold-specific binding protein, GolB, was recently identified, providing a unique opportunity for the study of the Au–S bond at the molecular level. We probed the mechanical strength of the gold–sulfur bond in GolB using single-molecule force spectroscopy. We measured the rupture force of the Au–S bond to be 165 pN, much lower than Au–S bonds measured on different gold surfaces (∼1000 pN). We further solved the structures of apo-GolB and Au(I)–GolB complex using X-ray crystallography. These structures showed that the average Au–S bond length in GolB is much longer than the reported average value of Au–S bonds. Our results highlight the dramatic influence of the unique biological environment on the stability and strength of metal coordination bonds in proteins. |
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disjustar
铁杆木虫 (正式写手)
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4楼2015-12-09 11:45:08
jnxybsf
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小云云。: 金币+6, ★★★很有帮助 2015-12-08 12:09:23
爱与雨下: 金币+1 2015-12-08 21:31:35
小云云。: 金币+6, ★★★很有帮助 2015-12-08 12:09:23
爱与雨下: 金币+1 2015-12-08 21:31:35
| 金硫配位键已在众多领域得到了广泛研究及应用，但对于该键的基础性质还有待研究。近来，一种含有特定金建的蛋白质GolB已被确定，这为在分子水平上研究金-硫键提供了独特的条件。我们用单分子力光谱检测了GOlB中金-硫键的机械强度。该金-硫键的断裂力为165pN,远小于不同金原子表面的金-硫键(约1000pN).我们进一步用x射线结晶法确定了apo-GolB and Au(I)–GolB复合物的结构，结果表明GolB中金-硫键的平均键长远大于所报道的值。研究结果强调了独特的生物环境对蛋白质中金属配位键的稳定性和强度的显著影响 我是外行，仅供参考 |
2楼2015-12-08 00:07:00
小云云。
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3楼2015-12-08 06:26:39
小云云。
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5楼2015-12-09 12:53:24
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