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[求助]
有做动物实验的大神吗？能帮我看看这篇论文的动物分组问题吗？
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Animal pairs were pseudorandomized into one of five conditions: Sham— control diet (n=5); Sham—high fat, high sugar diet (HFS; n=6); 2-vessel occlusion—control diet (2VO; n=8); 2VO-HFS diet (n=10); and 2VOt PA/CA (n=11). Animals in the Sham, HSF, 2VO, and 2VO-HSF groups were housed in pairs and a single condition was represented within a cage. The PA/CA condition consisted of a paradigm previously used in our laboratory3 and was implemented after 2VO surgery (Experiment 2). Animals in this condition were individually exposed to voluntary running wheels, 3 days/week and on alternate days were housed in pairs and exposed to cognitive activity, 3 days/week; the seventh day they were housed in pairs in the absence of PA or CA. Physical activity consisted of exposure to 36 cm diameter wheels for 24 hours a day. Cognitive activity consisted of modified Hebb–Williams maze (10010020 cm3),14 for 24 hours a day, with the maze configuration changed weekly to increase novelty. Additionally, novel objects and food rewards were scattered throughout the maze and the water bottle and food hopper were separated to encourage exploration. The PA/CA animals were housed in these conditions throughout the study. The PA/CA animals were housed in these conditions throughout the study except during behavioral testing and pair-housed in standard cages on the remaining day each week. 我查资料即使采用伪随机数分组，但是每组动物数量最起码是相等的呀，但是这篇论文好像是每组数量都是随机的。而且 Sham—control diet (n=5)的组数少于6，不是说是要具有统计学意义，数据个数不能少于6吗？求大神帮忙解惑！！！！ |
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