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After the solution was cooled to room temperature, the resulting solution was neutralised with 1 M NaOH and dialysed against deionised water using a dialysis bag with a MWCO of 8000 Da, giving rise to dialysable (outside the dialysis bag) sample and non-dialysable (inside the dialysis bag) sample. The methylated samples were hydrolysed with 2 M TFA (121 C, 2 h), reduced with NaBH4, and acetylated to convert into their partially methylated alditol acetates. The derivatives 求大神翻译 |
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