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Thus, the 7-fold difference observed between the kcat/KM (and KM) of O2 between three-domain laccases and SLAC might reflect an evolutionary adaptation to the O2-rich environment in which SLAC operates. The lower kcat/KM of SLAC might be brought about by a reduced accessibility of the TNC connected with the access channel to the TNC in the trimeric form of SLAC.36
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