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| As for the evolutionary history of the three-domain laccases, it may be that the evolution of ascorbate oxidase and other three-domain MCOs such as the laccases took separate, divergent paths or that, in the evolutionary process, subtle changes were incorporated in the primary coordination sphere of the TNC and also in the nearby protein environment by reorganizing the residues and the overall arrangement of the cupredoxin domains to provide a more efficient oxidase activity in laccases as compared to SLAC and hCp. |
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