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2Â¥: Originally posted by ·´Ó¦Á´ at 2015-10-13 19:30:19
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11Â¥: Originally posted by orangeiii at 2016-03-01 15:31:59
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What can I do for you?
BTW, how about your negative control without insert DNA, which can be used for confirming double digestion completely?
Maybe you can make the vector ends blunt. Then a single T will be added respectively to the 5¡ä ends of the vector with Taq DNA polymerase. After that a TA-cloning-like assay will be done, and the direction of inserted DNA can be selected by sequencing.

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12Â¥2016-03-01 22:50:31
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4Â¥: Originally posted by ·´Ó¦Á´ at 2015-10-14 20:10:44
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4Â¥: Originally posted by ·´Ó¦Á´ at 2015-10-14 20:10:44
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