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[求助]
电位滴定法测定酶与载体之间的吸附机理问题
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3.2. Adsorption mechanism To study the adsorption mechanism, zeta potentials at different pH between supports and lipase were measured, and the results are shown in Fig. 4. The isoelectric point (IEP) of magnetic silica nanoparticles is pH 2.7 similar to previous findings [39]. While for the magnetic silica coated with ionic liquids, IEP increased to pH 9.2. Ionic liquids changed surface electrokinetics of the magnetic supports from anionic to cationic, which is consistent with the findings that imidazolium cation of ionic liquids can form complex with negatively charged surface [40]. The resultant positive surface at neutral pH has strong affinity with negatively charged lipase, whose IEP is 4.5 [41]. The immobilization of lipase on magnetic nanoparticles supported ionic liquids was conducted by ionic adsorption.  DXGRVSVWPZ)DND}T]WLN{3W.png |
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2楼2015-10-09 14:21:10
