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看到有人求助这段英文翻译，可我却没有权限回帖，在此与各位交流，请多多指教！
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2.5. Analysis of the composition of SFP The HPLC method was used to identify the monosaccharides in SFP [11]. The sample (10 mg) of SFP was hydrolyzed with 2 mol/l H2SO4 (2 ml) at 100 ◦C for 5 h, and the excess acid was neutralized with barium carbonate. After standing overnight, the neutral products were separated by centrifugation at 3000 rpm for 10 min, 100 l supernatant was collected and reacted with 1- phenyl-3-methyl-5-pyrazolone, then subsequently separated by HPLC (Shimadzu LC-10AT VP, Japan) using a Packed column (4.6 × 250 mm) with detection at 250 nm. The mobile phase were consisted of 78% phosphate buffer solution (0.05 M, KH2PO4-NaOH, pH 6.9) and 22% acetonitrile (v/v), and the samples were eluted at a flow rate of 1.0 ml/min at 28 ◦C. In this study, l-Rha, d-Gal, d-GalA, d-Glu and Ara were used as references. 我觉得可以这样翻译：2.5.SFP组分的分析 高效液相色谱法常被用于鉴定SFP中的单糖，10ml的SFP样品与2ml浓度为2mol/ml的 H2SO4在100◦C的温度下水解5个小时，过量的酸则用碳酸钡中和掉。这样放置一个晚上后，以每分钟3000转的转速离心10min可除去中和产物，收集100ml的上清液与1-苯基-3-甲基-5-吡唑酮反应，然后用高效液相色谱仪(型号：Shimadzu LC-10AT VP, Japan)进行分离，填充柱规格为4.6 × 250 mm，在250nm的波长条件下测定。流动相组成为78%的磷酸盐缓冲液 (0.05 M, KH2PO4-NaOH,pH 6.9)和22%的乙腈溶液(v/v)，温度为28 ◦C、洗脱液流速1.0 ml/min洗脱样品。此次研究中，标准品为L-鼠李糖，D-氨基半乳糖，D-氨基半乳糖胺，花生四烯酸。 |
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