24小时热门版块排行榜

返回列表

【奖励】本帖被评价5次，作者yuan0806增加金币 4 个

yuan0806

铁杆木虫 (著名写手)

应助: 143 (高中生)
金币: 8257.7
帖子: 2057
在线: 1744.3小时
虫号: 989475

[资源] 核酸适体大牛谭蔚泓分析化学新作

核酸适体大牛谭蔚泓分析化学新作：Efficient Two-Photon Fluorescent Probe with Red Emission for Imaging of Thiophenols in Living Cells and Tissues

双光子荧光探针检测活细胞组织中的苯硫酚

Thiophenols, a class of highly toxic and pollutant compounds, are widely used in industrial production. Some aliphatic thiols play important roles in living organisms. Therefore, the development of efficient methods to discriminate thiophenols from aliphatic thiols is of great importance. Although several one-photon fluorescent probes have been reported for thiophenols, two-photon fluorescent probes are more favorable for biological imaging due to its low background fluorescence, deep penetration depth, and so on. In this work, a two-photon fluorescent probe for thiophenols, termed NpRb1, has been developed for the first time by employing 2,4-dinitrobenzene-sulfonate (DNBS) as a recognition unit (also a fluorescence quencher) and a naphthalene-BODIPY-based through-bond energy transfer (TBET) cassette as a fluorescent reporter. The TBET system consists of a D-π-A structured two-photon naphthalene fluorophore and a red-emitting BODIPY. It displayed highly energy transfer efficiency (93.5%), large pseudo-Stokes shifts upon one-photon excitation, and red fluorescence emission (λem = 586 nm), which is highly desirable for bioimaging applications. The probe exhibited a 163-fold thiophenol-triggered two-photon excited fluorescence enhancement at 586 nm. It showed a high selectivity and excellent sensitivity to thiophenols, with a detection limit of 4.9 nM. Moreover, it was successfully applied for practical detection of thiophenol in water samples with a good recovery, two-photon imaging of thiophenol in living cells, and tissues with tissue-imaging depths of 90–220 μm, demonstrating its practical application in environmental samples and biological systems.

回复此楼

» 本帖附件资源列表

欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com
附件 1 : liu2015_An_Efficient_Two-Photon_Fluorescent_Probe_with_Red_Emission.pdf

2015-09-02 10:13:25, 687.51 K