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zmm

木虫 (小有名气)

[求助] 外行请教免疫荧光染料问题 已有1人参与

想用荧光标记的抗体来检测细胞表面的抗原marker
发现有些抗体是两种荧光染料同时标记的
比如PE-Cy7 anti-mouse CD11b,PerCP-Cy5.5 anti-mouse B220
而有些就是一种荧光染料标记的
比如:AlexaFluor-488 anti-mouse CD3

想请教下,一种荧光标记和两种荧光标记的区别和目的各是什么呢?

谢谢!!
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sheepshowzz

铜虫 (初入文坛)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
zmm: 金币+10, ★★★★★最佳答案 2015-08-03 11:40:08
zmm: 金币+10, ★★★★★最佳答案 2015-08-03 11:40:43
??????l?????????????Ξ??.
???A??B??????????r, A?????l????????????????B?o????, ?B????????h??????.

Ξ?????
http://cmurdc.cmu.edu.tw/HVIS/5- ... owcytometer/Dye.htm

???, ?F????????????????????V, ????a?????V??B(Spectral overlap)????Ξ?????(Spillover), ?????f???Ξ?Ⅲ??????????r, ????????Ξ??????g?????_????}???????Q.
?@?????????d??????????о? ????W?a????(Compensation)...
http://www2.cmu.edu.tw/~cmcrdc/e ... ass=A&id=18
abcdogbitepig
2楼2015-08-03 11:07:05
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sheepshowzz

铜虫 (初入文坛)

??...????????v?????c?y??...
???????????????x??, ????????????x??_?^???M??.
?????f????????l?????????????Ξ??..
??????PE??PE-Cy5??PE-Cy7????l????496nm.
???s????a?????N?????????, 578??667??785nm.

http://www.bdbiosciences.com/tw/ ... sampledata/blue.jsp
abcdogbitepig
3楼2015-08-03 11:27:57
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zmm

木虫 (小有名气)

???????:
3?: Originally posted by sheepshowzz at 2015-08-03 11:27:57
??...????????v?????c?y??...
???????????????x??, ????????????x??_?^???M??.
?????f????????l?????????????Ξ??..
??????PE??PE-Cy5??PE-Cy7????l????496nm.
???s????a?????N?????? ...

???????????лл??????
4楼2015-08-03 11:39:39
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