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克拉克肯特

至尊木虫 (著名写手)

[求助] 求一句英文翻译,有点儿原理最好

To repair these two deletions, we divided F6 and F8 into two smaller fragments, F6-1\F6-2 and F8-1\F8-2, respectively. These four smaller fragments were obtained by PCR amplification and cloned into the pMD18-T vector for DNA sequencing. The correct DNA fragments were assembled into F6 and F8 using the modified method.
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baoshanqiu

至尊木虫 (著名写手)

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爱与雨下: 金币+1 2015-07-09 06:41:14
克拉克肯特: 金币+15, 翻译EPI+1, ★★★★★最佳答案 2015-07-10 18:37:56
为了修复这两个缺失,我们将F6和F8各自分成两个更小的片段,分别为F6-1\F6-2 和 F8-1\F8-2。用PCR扩增得到这四个更小的片段并克隆到pMD18-T 载体供DNA测序。用改进的方法将正确的DNA片段装配到F6和F8。

基本的是利用PCR扩增可以填补缺失的DNA片段。至于为什么要把片段分得更小,这要看上下文。可能与克隆操作有关(片段大小、酶切位点、测序等)
2楼2015-07-08 22:02:29
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