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[求助]
英译汉，要求正确流畅
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| In cells a portion of cytochrome c (cyt c) (15–20%) is tightly bound to cardiolipin (CL), one of the phospholipids constituting the mitochondrial membrane. The CL-bound protein, which has nonnative tertiary structure, altered heme pocket, and disrupted Fe(III)-M80 axial bond, is thought to play a role in the apoptotic process. This has attracted considerable interest in order to clarify the mechanisms governing the cyt c–CL interaction. Herein we have investigated the binding reaction of CL with the c-type cytochromes from horse heart and yeast. Although the two proteins possess a similar tertiary architecture, yeast cyt c displays lower stability and, contrary to the equine protein, it does not bind ATP and lacks pro-apoptotic activity. The study has been performed in the absence and in the presence of ATP and NaCl, two compounds that influence the (horse cyt c)-CL binding process and, thus, the pro-apoptotic activity of the protein. The two proteins behave differently: while CL interaction with horse cyt c is strongly influenced by the two effectors, no effect is observed for yeast cyt c. It is noteworthy that NaCl induces dissociation of the (horse cyt c)–CL complex but has no influence on that of yeast cyt c. The differences found for the two proteins highlight that specific structural factors, such as the different local structure conformation of the regions involved in the interactions with either CL or ATP, can significantly affect the behavior of cyt c in its reaction with liposomes and the subsequent pro-apoptotic action of the protein. |
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| 细胞中部分细胞色素c(cyt c) (15–20%)与心磷脂(CL)紧密结合。该心磷脂是构成线粒体膜的磷脂之一。CL-结合蛋白具有无活性的三级结构、改变的血红素袋和破坏的Fe(III)-M80柱向键，已知在凋亡过程中起一定作用。这在试图弄清有关cyt c–CL相互作用机理的研究中引起了相当的关注。在此我们研究了CL与源自马心和酵母的c-型细胞色素的结合反应。虽然两种蛋白具有相似的三级结构，但酵母cyt c稳定性低，与马蛋白相反，它不结合ATP且没有促凋亡活性。实验在有和没有ATP 和 NaCl的条件下进行。这两种化合物影响（马 cyt c）-CL的结合过程进而影响该蛋白的促凋亡活性。两种蛋白具有不同的特性：两种效应剂强烈影响CL与马cyt c的相互作用，但不影响酵母cyt c。值得注意NaCl 诱导(马 cyt c)–CL复合物解离但不影响对应的酵母cyt c。从两种蛋白观察到的差异强调特定的结构因素，诸如参与同 CL 或 ATP相互作用的局部结构形态差异，会明显地影响cyt c在与脂质体反应中的特性并进而影响该蛋白的促凋亡活性。 |
2楼2015-06-24 02:14:25
