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Ectoine belongs to the family of compatible solutes, which are known to contribute mainly to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition, the cell’s essential functions are maintained under difficult conditions like high salinity, heat, or aridity stress. Biosynthesis of ectoine has been found in halophilic and halotolerant microorganisms. We showed that the methanotrophic bacterial consortium (MBC) isolated from coalbed rocks from coalmine Bogdanka (Poland) and resistant to extreme environmental conditions (low content of moisture) was able to synthesize ectoine. MBC was cultured in mineral nitrate mineral salts medium supplied with NaCl at atmospheric air enriched with 10% of methane. The levels of methanotrophic activity were determined by the gas chromatography technique (943.05 ± 30.73 – 94.14 ± 0.85 μ M CH 4 gDW –1 day –1 ) and the biomass concentration of MBC was evaluated based on OD 600 ,as well as biosynthesis of ectoine in relation to the salinity (0–5% NaCl) of the medium. The levels of ectoine tested by NIR measurements ranged from 1.33 ± 0.10 mg gDW –1 to 0.42 ± 0.08 mg gDW –1 depending on the salinity of the solution. In addition, we identified MBC based on the pmo A gene. |
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四氢嘧啶属于可混溶溶质。已知这类溶质在细胞以恒定膨压适应渗透应力时起主要作用。此外，在生存困境如高盐、热或干旱胁迫时细胞的基本功能得以维持。已发现嗜盐和耐盐的微生物可生物合成四氢嘧啶。我们发现从波格丹卡（波兰）煤矿的煤床岩层分离的甲烷营养菌群（MBC)能抗极端环境条件（低湿度），能合成四氢嘧啶。将MBC用添加NaCl的矿物硝酸盐矿物盐培养基于富含10%甲烷的气体下培养。甲烷营养活性用气相色谱法测定（943.05 ± 30.73 – 94.14 ± 0.85 μ M 甲烷/克干样/天),MBC的生物量浓度用OD 600表示，并确定了四氢嘧啶生物合成与培养基中盐浓度（0-5%NaCl)的关系。四氢嘧啶浓度用近红外法测定为 1.33 ± 0.10 毫克/克干样 至 0.42 ± 0.08 毫克/克干样，取决于溶液中盐浓度。 另外，我们还以特异甲烷加单氧酶A 鉴定MBC. |
2楼2015-06-11 23:30:37