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bxzc123

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The genome context of the ect genes was explored to identify proteins that are functionally associated with thesynthesisof ectoines;thespecialized aspartokinaseAsk_Ect and theregulatory protein EctR. This comprehensive in silico analysis was coupled with the biochemical characterization of ectoine hydroxylases from microorganisms that can colonize habitats with extremes in salinity (Halomonas elongata), pH (Alkalilimnicola ehrlichii,Acidiphilium cryptum), or temperature (Sphingopyxis alaskensis, Paenibacillus lautus) or that produce hydroxyectoine very efficiently over ectoine (Pseudomonasstutzeri).These six ectoine hydroxylasesall possesssimilar kinetic parametersfor their
substrates but exhibit different temperature stabilities and differ in their tolerance to salts. We also report the crystal structure of the Virgibacillus salexigens EctD protein in its apo-form, thereby revealing that the iron-free structure exists already in a pre-set configuration to incorporate the iron catalyst. Collectively, our work definesthe taxonomic distribution and salient biochemical properties of the ectoine hydroxylase protein family and contributes to the understanding of its
structure.

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baoshanqiu

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°®ÓëÓêÏÂ: ½ð±Ò+1 2015-06-11 06:51:21
bxzc123: ½ð±Ò+50, ·­ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú 2015-06-11 10:40:57
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