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Ectoine and hydroxyectoine are well-recognized members of the compatible solutes and are widely employed by microorganisms as osmostress protectants. The EctABC enzymes catalyze the synthesis of ectoine from the precursor L-aspartate-b-semialdehyde. A subgroup of the ectoine producers can convert ectoine into 5-hydroxyectoine through aregion-selective and stereospecific hydroxylation reaction. Thiscompatible solute possessesstress-protective and function-preserving propertiesdifferent from thoseof ectoine.Hydroxylation of ectoineiscarried out by the EctDprotein,amember of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily. We used the signatureenzymesfor ectoine(EctC)and hydroxyectoine(EctD)synthesisin databasesearchesto assessthetaxonomicdistribution of potential ectoine and hydroxyectoine producers. Among 6428 microbial genomes inspected, 440 species are predicted to produce ectoine and of these, 272 are predicted to synthesize hydroxyectoine as well. Ectoine and hydroxyectoine genes are found almost exclusively in Bacteria. |
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bxzc123: 金币+50, 翻译EPI+1, ★★★★★最佳答案 2015-06-11 11:11:09
bxzc123: 金币+50, 翻译EPI+1, ★★★★★最佳答案 2015-06-11 11:11:09
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Ectoine and hydroxyectoine are well-recognized members of the compatible solutes and are widely employed by microorganisms as posmostress protectants. 四氢嘧啶和羟基四氢嘧啶是公认的相容性溶质，并广泛运用于微生物领域，作为高渗胁迫保护剂。 The EctABC enzymes catalyze the synthesis of ectoine from the precursor L-aspartate-b-semialdehyde. A subgroup of the ectoine producers can convert ectoine into 5-hydroxyectoine through aregion-selective and stereospecific hydroxylation reaction. EctABC酶能催化，从前驱体左旋天冬氨酸b-半醛中合成四氢嘧啶。 有一个四氢嘧啶生产者的小组能通过一种具有区域指向性和立体定向性的羟基化反应，将四氢嘧啶转变成5-羟基四氢嘧啶。 This compatible solute possessesstress-protective and function-preserving properties different from those of ectoine.Hydroxylation of ectoine is carried out by the EctD protein,a member of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily. 这种相容性溶质，具有不用于四氢嘧啶的，压力保护和功能保留的特性。通过加入EctD蛋白质这种属于非血红素铁和酮戊二酸的双加氧酶家族的物质，能够催化四氢嘧啶的羟基化反应。 We used the signature enzymes for ectoine(EctC)and hydroxyectoine(EctD)synthesis in database searches to assess the taxonomic distribution of potential ectoine and hydroxyectoine producers. 我们运用标记酶来催化EctC（四氢嘧啶）和EctD（羟基四氢嘧啶）的合成反应，在数据库检索，评估出潜在的EctC和EctD生产者的分类分布。 Among 6428 microbial genomes inspected, 440 species are predicted to produce ectoine and of these, 272 are predicted to synthesize hydroxyectoine as well. Ectoine and hydroxyectoine genes are found almost exclusively in Bacteria. 在一共调查的6428个微生物基因组中，有440种被认为是可以生产四氢嘧啶的，而在这440钟潜在生产者中，又有272种被认为可以生产羟基四氢嘧啶。四氢嘧啶和羟基四氢嘧啶基因，几乎只在细菌中有被发现过。 |
2楼2015-06-11 11:04:23