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宋伟同学

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[求助] 请问免疫共沉淀后,如何把吸附的蛋白和琼脂糖珠分离开来。谢谢。已有1人参与

不是吸附柱,就是琼脂糖珠,沉淀玩如何把目的蛋白再弄下来。谢谢。
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℃lizzy

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【答案】应助回帖

Subsequently, the beads were washed three times each with 500μl of cold lysis buffer. Immunoprecipitates were eluted from the beads by adding 30μl of SDS-PAGE sample buffer and heating for 20 min at 75°C. The eluates (15μl) were analyzed by Western blotting.
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