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[求助]
谁帮我看下这段话啊，重金求助，改得越仔细越好，改得好追加金币
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To avoid lipase deactivation by methanol in the enzymatic transesterification, a two-step biocatalytic process for biodiesel production from unrefined jatropha oil was developed. The unrefined jatropha oil was first hydrolyzed to free fatty acids (FFAs) by the commercial enzyme Candida rugosa lipase. A maximum FFAs yield of 90.3 % was achieved after 8 h at a water to WCO ratio of 0.75:1 (w/w), 2%(w/w) of lipase and 40℃. After the hydrolysis, the FFAs were separated and esterified with methanol to biodiesel by using the whole-cell Rhizopus oryzae IFO4697 immobilized within biomass support particles (BSPs) as a whole-cell biocatalyst. Molecular sieves (3 Å  were added into the reaction mixture to online remove the produced water. The maximum fatty acid methyl ester (FAME) yield reached 88.6% after 42 h at a molar ratio of methanol to FFAs 1.2:1, 60 %(w/w) of molecular sieves (3 Å and 35℃. C. rugosa lipase and R. oryzae whole cell catalyst in the process showed excellent reusability, retaining 89% and 79% productivity, respectively, even after six batches. This novel process, combining the advantages of enzyme and whole cell catalysts, saved the consumption of commercial enzyme and avoids enzyme deactivation by methanol. |
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genhunter
至尊木虫 (著名写手)
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Its well written! only minor corrections are needed. To avoid lipase deactivation by methanol in the enzymatic transesterification, a two-step biocatalytic process for biodiesel production from unrefined jatropha oil was developed. The unrefined jatropha oil was first hydrolyzed to free fatty acids (FFAs) by the commercial [enzyme] Candida rugosa lipase. A maximum FFAs yield of 90.3 % was achieved after 8 hrs at a water to WCO (?什么) ratio of 0.75:1 (w/w), 2%(w/w) of lipase，under 40℃. After the hydrolysis, the FFAs were separated and esterified with methanol to biodiesel by using the whole-cell Rhizopus oryzae IFO4697, immobilized within biomass support particles (BSPs) as a whole-cell biocatalyst. Molecular sieves (3 Å were added into the reaction mixture to remove water as it was produced. The maximum yield for fatty acid methyl ester (FAME) reached 88.6% after 42 hrs at a molar ratio of methanol to FFAs of 1.2:1, 60 %(w/w) of molecular sieves (3 Å , under 35℃. C. rugosa lipase and R. oryzae whole cell catalyst in the process showed excellent reusability, retaining 89% and 79% yields, respectively, even after six batches of reactions. This novel process, combining the advantages of the efficiency and mild reaction conditions catalyzed by the enzyme and whole cell catalysts, significantly cuts the cost of the commercial enzyme and avoids the deactivation enzyme by methanol. |
8楼2015-05-31 19:41:18
鸟子
金虫 (正式写手)
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2楼2015-05-31 18:55:59
3楼2015-05-31 19:24:23
genhunter
至尊木虫 (著名写手)
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
welzgx: 金币+50, 翻译EPI+1, ★★★★★最佳答案, 虽然误解了意思，但感谢用心，给一半吧。我要的是看有没有问题和修改 2015-05-31 19:31:30
welzgx: 金币+50, 翻译EPI+1, ★★★★★最佳答案, 虽然误解了意思，但感谢用心，给一半吧。我要的是看有没有问题和修改 2015-05-31 19:31:30
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今晚最后一个。 为了避免脂肪酶在酶法酯交换反应中被甲醇失活，开发了两步生物催化工艺从未精制的麻疯树油生产生物柴油。 未精制的麻疯树油首先用购买的褶皱假丝酵母脂肪酶水解为游离脂肪酸（FFA）。在水和WCO重量比为0.75:1, 2%（w/w）脂肪酶和40℃下，8 h后得到最高产量为90.3%。水解后，游离脂肪酸进行了分离和用米根霉细胞固定化成微生物载体颗粒内（BSP）, 作为完整细胞生物催化剂，和甲醇脂化制备成生物柴油。分子筛（3Å）加入到反应混合物中除去产生的水。反应42小时以后，甲醇和游离脂肪酸的摩尔比为1.2:1，60%（w/w）分子筛（3Å）和35℃下, 最高的脂肪酸甲酯（FAME）产量达到88.6%。 过程中褶皱假丝酵母脂肪酶和米根霉整细胞催化剂的显示出良好的可重用性，即使经过六次后，仍可以保持分别为89%和79%的催化能力。这个新工艺，结合酶和整细胞催化剂的优点，节省了需要购买的酶的消耗和避免甲醇导致酶的失活。 |
4楼2015-05-31 19:28:17
5楼2015-05-31 19:30:09
genhunter
至尊木虫 (著名写手)
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6楼2015-05-31 19:30:16
7楼2015-05-31 19:36:45
genhunter
至尊木虫 (著名写手)
- 翻译EPI: 387
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- 金币: 9757.4
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- 注册: 2013-07-22
- 专业: 肿瘤化学药物治疗
9楼2015-05-31 19:45:19
10楼2015-06-04 16:37:08