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xiaowanzi9新虫 (正式写手)
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Finally, we investigated direct effects of fumaric acid esters on the inhibitor of Nrf2, Keap1 in vitro. Mass spectroscopy revealed a covalent modification at the cysteine residue 151 of the Keap1 protein after monomethylfumarate treatment (Fig. 5G). The matched Keap1 peptides (peptide score 47) from a SpectrumMill database search covered 95% of the rat Keap1 protein sequence. After stimulation with monomethylfumarate, a 130 Da mass increase of the Keap1 tryptic peptide CVLHVMNGAVMYQIDSVVR was observed. The mass increase of 130 Da is consistent with a Michael addition of a free cysteine sulphydryl group across the monomethylfumarate double bond. The MS/MS spectrum of the peptide confirms this modification at cysteine 151 (Fig. 5G). The assignment of 130 Da to the monomethylfumarate modification is consistent (within 0.14 mmu) with a chemical formula of C5H6O4 (130.02661 exp. and 130.02647 cal.). The ion intensity ratio of the modified and unmodified peptides are 69 and 31%, respectively. The monomethylfumarate induced 130 Da modification was also observed––to a much lower extent––in other cysteine-containing Keap1 peptides (Table 2). |
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武汉一心一译 
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xiaowanzi9: 金币+30, 翻译EPI+1 2015-05-25 16:26:09
xiaowanzi9: 金币+30, 翻译EPI+1 2015-05-25 16:26:09
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Finally, we investigated direct effects of fumaric acid esters on theinhibitor of Nrf2, Keap1 in vitro. 最后，我们研究了 反丁烯二酸酸酯对Keap1体外，Nrf2的抑制剂的直接作用 Mass spectroscopy revealed a covalent modification at the cysteineresidue 151 of the Keap1 protein after monomethylfumarate treatment (Fig. 5G). The matched Keap1 peptides (peptide score 47) from a Spectrum Mill database search covered 95% of the rat Keap1 protein sequence. 质谱分析表明， 运用富马酸单甲酯进行处理后，会形成一种，对Keap1蛋白质的半胱氨酸残基151的共价修饰（如图5G）。通过光谱数据库搜索获得的与之匹配的Keap1多肽（肽分为47）， 覆盖了95%的老鼠Keap1蛋白质序列。 After stimulation with monomethylfumarate,a 130 Da mass increase of the Keap1 tryptic peptide CVLHVMNGAVMYQIDSVVR was observed. The mass increase of 130 Da is consistent with a Michael addition of a free cysteine sulphydryl group across the monomethylfumarate double bond. 运用富马酸单甲酯进行刺激后，可以观察到，Keap1胰蛋白酶肽，会产生130 Da级的质量增加。该130Da的质量增加，与自由半胱氨酸巯基集团杂交富马酸单甲酯双键，的迈克尔加成反应一致。 The MS/MS spectrum of the peptide confirms this modification at cysteine 151 (Fig. 5G). The assignment of 130 Da to the monomethylfumarate modification is consistent (within 0.14 mmu) with a chemical formula of C5H6O4 (130.02661 exp. and 130.02647 cal.). The ion intensity ratio of the modified and unmodified peptides are 69 and 31%, respectively. 缩氨酸的串联式质谱法，确定了半胱氨酸151（如图5G） 修饰。130 Da分配到富马酸单甲酯修饰，与（在0.14 mmu内的）C5H6O4的化学式 （130.02661 exp. and 130.02647 cal.）一致。修饰/未修饰的肽的相对离子强度比分别为69% 和 31%。 The monomethylfumarate induced 130 Da modification was also observed––to a much lower extent––in other cysteine-containing Keap1 peptides (Table 2). 同时，也可以观察到富马酸单甲酯有法的130 Da的修饰。在含其它半胱氨酸的Keap1多肽中，其修饰程度更低。（如表二所示） |
3楼2015-05-25 15:54:36