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vc小小子

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标题   鼠伤寒沙门菌SL1344Δcrp缺失株对HeLa细胞黏附和侵入的检测
摘 要
鼠伤寒沙门菌的黏附和侵入是引起大多数感染的第一步,同时又是评价该病原体毒力的重要指标之一。为了研究鼠伤寒沙门菌SL1344及crp缺失株SL1344Δcrp对HeLa细胞黏附和侵入,首先分别对两株菌株进行活化、稀释涂板,过夜培养后计数;对HeLa细胞复苏、传代、冻存及计数。随后对鼠伤寒沙门菌SL1344和SL1344Δcrp菌株分别进行黏附试验和侵入试验。实验结果显示,在黏附试验中,SL1344菌株的黏附率为0.155%,SL1344Δcrp株的黏附率为0.12%,差异显著(P<0.05)。在侵入试验中,SL1344菌株的侵入率为0.1%,而SL1344Δcrp株的侵入率为0.08%,两者的侵入率相比差异显著(P<0.05)。总体而言,与SL1344亲本菌株相比,crp缺失株对HeLa细胞的黏附率与侵袭率均发生降低,表明crp缺失株的致病力发生了减弱,该结果为研制安全有效的鼠伤寒沙门菌疫苗以及疫苗载体奠定了基础。
关键词:鼠伤寒沙门菌,crp,黏附,侵入

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genhunter

至尊木虫 (著名写手)

【答案】应助回帖

建议笔者先修饰一下,去除不必要的实验细节(话化、稀释涂板,过夜培养后计数;对HeLa细胞复苏、传代、冻存及计数之类,把中文部分搞顺了,然后再求助。
离成功还差一点
2楼2015-05-22 10:57:44
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genhunter

至尊木虫 (著名写手)

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vc小小子: 金币+30, ★★★很有帮助 2015-05-23 21:08:31
那我帮你改了?
标题   鼠伤寒沙门菌SL1344Δcrp缺失株对HeLa细胞黏附和侵入的检测
摘 要
鼠伤寒沙门菌的黏附和侵入是引起大多数感染的第一步,同时又是评价该病原体毒力的重要指标之一。为了研究鼠伤寒沙门菌对HeLa细胞黏附和侵入机制的研究,分别对两株菌株SL1344和SL1344Δcrp菌株分别进行黏附试验和侵入试验。实验结果显示,在黏附试验中,SL1344菌株的黏附率为0.155%,SL1344Δcrp株的黏附率为0.12%,差异显著(P<0.05)。在侵入试验中,SL1344菌株的侵入率为0.1%,而SL1344Δcrp株的侵入率为0.08%,两者的侵入率相比差异显著(P<0.05)。总体而言,与SL1344亲本菌株相比,crp缺失株对HeLa细胞的黏附率与侵袭率均发生降低,表明crp缺失株的致病力发生了减弱,该结果为研制安全有效的鼠伤寒沙门菌疫苗以及疫苗载体奠定了基础。
关键词:鼠伤寒沙门菌,crp,黏附,侵入
Adhesion and Invasion Salmonella typhimurium SL1344 Δcrp Mutant Strain on HeLa Cells  
Abstract
Adhesion and invasion of Salmonella typhimurium  constitute the first step for  most of the infection, and is also one of the important indicators that is related to  the virulence of the pathogen . In order to study the mechanism of adhesion and invasion of Salmonella typhimurium to HeLa cell,  Salmonella typhimurium SL1344 and Δcrp Mutant strains were tested. Experimental results show that for the adhesion tests, the adhesion rate for strain SL1344  was 0.155% and for strain SL1344  Δcrp was 0.12%, the difference was significant (P < 0.05). For the invasive tests, strain SL1344 showed an invasion rate of 0.1%, and SL1344  Δcrp had an invasion rate of 0.08%, the difference between both rates were significant (P < 0.05). Overall, compared with the parent strain of SL1344, the Δcrp strain shows reduced adhesion and invasion rates to HeLa cells, indicating that SL1344 Δcrp strain had a weakened pathogenicity. The results may lay the foundation for the development of safe and effective  vaccine for Salmonella typhimurium as well as new vaccine vectors .
Keywords: Salmonella typhimurium, CRP, adhesion, invasion
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3楼2015-05-22 14:23:34
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