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Adhesion and Invasion Salmonella typhimurium SL1344 ¦¤crp Mutant Strain on HeLa Cells  
Abstract
Adhesion and invasion of Salmonella typhimurium  constitute the first step for  most of the infection, and is also one of the important indicators that is related to  the virulence of the pathogen . In order to study the mechanism of adhesion and invasion of Salmonella typhimurium to HeLa cell,  Salmonella typhimurium SL1344 and ¦¤crp Mutant strains were tested. Experimental results show that for the adhesion tests, the adhesion rate for strain SL1344  was 0.155% and for strain SL1344  ¦¤crp was 0.12%, the difference was significant (P < 0.05). For the invasive tests, strain SL1344 showed an invasion rate of 0.1%, and SL1344  ¦¤crp had an invasion rate of 0.08%, the difference between both rates were significant (P < 0.05). Overall, compared with the parent strain of SL1344, the ¦¤crp strain shows reduced adhesion and invasion rates to HeLa cells, indicating that SL1344 ¦¤crp strain had a weakened pathogenicity. The results may lay the foundation for the development of safe and effective  vaccine for Salmonella typhimurium as well as new vaccine vectors .
Keywords: Salmonella typhimurium, CRP, adhesion, invasion
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