至尊木虫 (知名作家)
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【答案】应助回帖
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bxzc123: 金币+100, 翻译EPI+1, ★★★很有帮助 2015-04-23 21:21:54
Based on these facts,Yan et al. [31] reported the microbial transformation of 3-hydroxy-5,6-cyclo propano cholestane-an alter route to 6-methyl steroids. Incubation of 3β-hydroxy-5, 6 α-cyclo propano-5 α-cholestane (6)with Mycobacterium sp. (NRRL B-3805) gave three metabolites, 5,6 α-cyclo propano-5 α-androsta-3, 17-dione (7), 3β-hydroxy-5,6 α-cyclo propano-5 α-androsta-17-one (8) and androsta-4-ene-3,17-dione (5). Fermentation of 3β-hydroxy-5, 6β-cyclo propano-5β-cholestane (9) with Mycobacterium sp. afforded the metabolite 5,5, 6β-cyclo propano-5β-androsta-3, 17-dione (10) and 3 α-hydroxy-5, 6β-cyclo propano-5β-androsta-17-one (11). Similarly, incubation of 3β-hydroxy-5, 6 α-cyclo propano-5 α-cholest-7-ene (12) with Mycobacterium sp. yielded a mixture of side chain cleaved 17-keto steroids as the major products, viz. the cyclopropyl eliminated product 5, 5, 6 α-cyclo propano-5 α-androst-7-ene-3, 17-dione (13), 3β-hydroxy-5, 6α-cyclo propano-5α-androst-4-ene-17-one (14), 5, 6 α-cyclo propano-5 α-androst-8(14)-ene-3, 7, 17-trione (15), 5, 6 α-cyclo propano-5 α-androst-8-ene-3, 7, 17-trione (16) and 6α, 7α-cyclo propano androst-4-ene-3,17-dione (17) as described in Scheme 2.
基于这些事实，Yan等【[31】报道的3-羟基-5,6-环丙醇胆甾烷微生物转化为6-甲基类固醇的不同途径。将3β羟基5,6α-环丙醇-5α-胆甾（6）与结核分枝杆菌（NRRL B-3805）孵育，得到3个代谢产物：5,6-α-环丙醇-5α-雄甾3,17二酮（7），3β羟基-5,6-α-环丙醇-5α-雄17酮（8）和雄甾-4-烯-3,17-二酮（5）。3β羟基-5-，6β-环丙醇-5β-胆甾（9）与结核分枝杆菌发酵，得到代谢物5,5，6β-环丙醇-5β-雄甾3,17二酮（10）和3α羟基-5，6β-环丙醇-5β-雄甾-17-酮（11）。类似地，如方案2中所述，3β羟基5,6α-环丙醇-5α-胆甾-7-烯（12）与结核分枝杆菌孵育，得到的主要产物是侧链17-酮类固醇侧链裂解混合物，即环丙基消除产物5，5，6α-环丙醇-5α-雄甾-7-烯-3，17-二酮（13），3β羟基-5，6α-环丙醇-5α-雄-4-烯-17酮（14），5，6α-环丙醇-5α-雄甾-8（14）-烯-3,7，17-三酮（15），5，6α-环丙醇-5-α-雄甾-8-烯-3，7，17三酮（16）和6α，7α-环丙醇，雄-4-烯-3,17-二酮（17）。 |
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