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The NCBI nr database has a large number of immunoglobulin sequences predicted from the GenBank. A total of 128 proteins were identified only in the immunoprecipitate from the HIV-E sample set. Interestingly, 90 of these proteins were immunoglobulin variable regions, and five of them were immunoglobulins against HIV-1 envelope proteins (Figure 2). We also identified glyoxalase double mutant, carbonic anhydrase, cold agglutinin, and CD47. The other 36 proteins were either immunoglobulins without region specification or unnamed protein products. Next, we analyzed the nitrated peptides to confirm that the identified proteins were nitrated. |
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