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malj09

新虫 (小有名气)

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虫号: 2188097
注册: 2012-12-15
专业: 药物化学

[交流] 药理学高通量筛选中的一些词汇----当合成遇到药理的时候痛苦就来了，，，，急求赐教

The data from six HTS campaigns were selected for
analysis (SI Table S2). All HTS campaigns discussed in this
paper displayed an acceptable signal-to-noise ratio as
judged by the Z0
values
17
being greater than 0.5 in each
assay event. The six HTS campaigns we selected employed
AlphaScreen technology (PerkinElmer), which we have
found to be particularly robust and reproducible. In these
campaigns, rigorous compound triaging through counter-
screens, secondary screens, and finally direct binding assess-
ment by surface plasmon resonance is routinely undertaken.
However, for the purposes of this current study, we delibe-
rately selected the primary hit set (single point inhibition) as
that which represented the least-processed set for each assay.
Rather, we controlled for experimental aberration through
the use ofmultiple (six) sets of primary screening hits. Primary
hit selection criteria are given in SI Table S2.We interrogated
the primary screening hits as follows. All library compounds
were binned according to the number of assays in which they
registered as hits according to those criteria for each particular
assay. As a general rule, compound screening concentrations
of between 10 and 30 μM were deployed. The results are
shown in Table 2

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