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PBS配方
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| 请教 PBS液10mM配制方法,非常感谢,急!网上查了有好几个配方,不知道哪个正确了, |
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这是我从wikipedia上查的，应该比较准。 Phosphate buffered saline（PBS） There are many different ways to prepare PBS. Some formulations do not contain potassium, while others contain calcium or magnesium. One of the most common preparations is described below. A 10 liter stock of 10x PBS can be prepared by dissolving 800 g NaCl, 20 g KCl, 144 g Na2HPO4 and 24 g KH2PO4 in 8 L of distilled water, and topping up to 10 L. The pH is ~6.8, but when diluted to 1x PBS it should change to 7.4. When making buffer solutions, it is good practice to always measure the pH directly using a pH meter. If necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide. On dilution, the resultant 1x PBS should have a final concentration of 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, and a pH of 7.4. 如配100ml: 0.8 g NaCl, 0.02g KCl, 0.144 g Na2HPO4 and 0.024 g KH2PO4 ,再加水至90ml，0.1%NaOH调PH=7.4，定容100ml。 |
2楼2008-07-03 11:42:32