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After reaction with NHS-PEG-MAL, the peak area at the binding energy of 286.4 eV increases (Figure 1c and Table 2), and a new peak at the binding energy of 532.7 eV appears (Figure 2c and Table 2) because the introduced PEG chains contain abundant C-O bonds. In the C1s spectrum, the peak area at the binding energy of 286.4 eV keeps increasing after the covalent binding of RGD-containing peptides because of the contribution of C-N and C-O bonds in the peptides (Figure 1d and Table 2). In the O1s spectrum, the peak areas at the binding energy of 531.3 eV increase significantly (Figure 2d and Table 2) because the grafted RGD-containing peptides contain abundant N-CdO bonds. Taken together, XPS results of different PHBV films indicate the successful binding of RGD-containing peptides to ammonia plasma-treated PHBV surfaces through the cross-linker of bifunctional PEG. |
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