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The microbial transformations of testosterone and testosterone heptanoate by four fungi: Absidia griseolla var. igachii PTCC 5260, Acremonium chrysogenu PTCC 5271, Fusarium fujikuroi PTCC 5144, and Fusarium solani complex PTCC 5285 were investigated for the first time. Incubation of testosterone heptanoate with F. fujikuroi and F. solani yielded three metabolites, which were isolated and characterized as testosterone, androst-4-ene-3,17-dione, and 6b-hydroxy testosterone. 6b-Hydroxy testosterone was the major metabolite obtained from testosterone heptanoate biotransformation by two fungal species. A. griseolla and A. chrysogenu produced 14 a -hydroxy testosterone as major metabolite, together with testosterone and 6b-hydroxy testosterone in lower yields. The biotransformation of testosterone by F. fujikuroi and A. griseolla was also investigated in order to examine the influence of the ester group on the course of transformation. Androst-4-ene-3,17-dione was only identified in the biotransformation of testosterone by F. fujikuroi. The same product was observed in incubation of testosterone by A. griseolla, together with 14 a -hydroxy testosterone in very low yield. Furthermore, time course study was also carried out in order to examine the formation of metabolites as a function of time, which was determined by HPLC. The structures of compounds were determined by their comprehensive spectroscopic analysis and comparison with literature data. |
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| 四种真菌对睾酮和庚酸睾酮的微生物转化：首次对犁头霉属griseolla var. igachii PTCC 5260、枝顶孢属chrysogenu PTCC 5271、链孢霉fujikuroi PTCC 5144和腐皮镰孢复合种PTCC 5285进行了研究。庚酸睾酮用镰刀恶苗和茄病镰刀菌培育得到三种代谢产物,经分离并表征为睾酮、雄甾-4-烯-3,17-二酮和6b-羟基睾酮。6b -羟基睾酮是由两种真菌生物转化庚酸睾酮得到的主要代谢产物。 A. griseolla和A. chrysogenu产生了作为主要代谢产物的14a-羟基睾酮还有较少的睾酮和6b-羟基睾酮。 为了检验酯基对转化过程的影响，还调查了用F.fujikuroi（镰刀恶苗）和A.griseolla对睾酮的生物转化。 在F. fujikuroi（镰刀恶苗）对睾酮的生物转化中鉴别出只有雄甾- 4 -烯3,17 -二酮。 在用A. griseolla对睾酮的培育中观察到相同产物还有极少量的14 a -羟基睾酮。此外,为了考查代谢产物随时间函数的形成情况，还进行了时程研究，通过高效液相色谱进行测定。通过对化合物的广泛的光谱分析并与文献资料对比确定了它们的结构。 |
2楼2015-03-31 10:00:44
