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Additionally, the reaction of the maleimide group with primary amines also occurs at a neutral pH level, although it is much slower than the reaction with sulfhydryls. During the 5 days of culture, some amount of proteins from the culture mediummay also chemically bind to the end of PEGby the reaction of maleimide groups with primary amines on proteins, which in turn promotes cell growth. The above explanation is supported by the protein-resistant properties of various modified PHBV films, which will be discussed below. Nevertheless, the final obtained RGD-modified PHBV films can significantly improve the cellular compatibility because of the RGD-containing peptides based on the investigation of cell morphology and cell viability. |
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