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From this proof of principle study several potential benefits from metabolomics for biomarker discovery and environmental risk assessment became evident: (i) Metabolites are often the first to react to stressors (Viant 2007). This became evident in the time-related patterns of metabolites detected in this study, as selected metabolites showed earlier responses in comparison to algal growth. (ii) The parallel analysis of algal growth parameters (cell volume) and metabolite composition indicates that metabolomics can support interpretation of well established toxicological assessment parameters. (iii) The study revealed multiple metabolic markers, responding to exposure, providing additional observation parameters to traditional endpoints in phytotoxicity assessment. (iv) Understanding toxicantinduced deviations from developmental metabolic trajectories may help to discriminate between primary and secondary toxic effects.

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