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[求助]
BCBI 提交序列，回信问题如何处理？？
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我向NCBI提交基因序列，三个月过去了，今天回信说我的两个序列5‘端有一个阅读框位移，什么意思啊，教我如何是好，求！ 回信如下： e are currently processing your GenBank records KP168713, KP218513 and have come across an issue that requires your attention. Upon doing an alignment of your sequences, we noticed that there is a reading frame shift in both sequences near their 5' ends: KP168713 1 GGTCAACAAAATCATAAAGATATTGGTATACTTTATTTTATTTTTGGAATCT KP218513 1 GGTCAACAAAATCATAAAGATATTGGAACTTTATATTTTATTTTTGGGGCTT 197110089 1 GGTCAACAAA-TCATAAAGATATTGGAATTTTATATTTTATTTTTGGGATTT 586695063 2 GGTCAACAAA-TCATAAAGATATTGGAATTTTATATTTTATTTTTGGGATTT 575503652 25 TCAACAAA-CCACAAAGACATTGGAACTTTGTATTTCATCTTTGGGGCTT 261876073 1 AA-CCATAAAGATATTGGAACTTTATACTTTATTTTTGGGGCTT 261876033 1 AA-CCATAAAGATATTGGAACATTATATTTTATTTTTGGGGCTT 45774897 25 AA-TCATAAAGATATTGGAATATTATATTTTATTTTTGGAATCT 51922073 25 AA-TCACAAAGATATTGGAATGCTTTATTTTATTTTTGGAATTT ^^^ If the nucleotide sequences are incorrect, send us the corrected sequences, and we will replace the current sequences in your submission. If the sequences are correct, please let us know if you have any evidence that these frame shifted proteins are translated. |
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2楼2017-06-20 09:48:14