| 查看: 467 | 回复: 1 | ||
[求助]
BCBI 提交序列，回信问题如何处理？？
|
|
我向NCBI提交基因序列，三个月过去了，今天回信说我的两个序列5‘端有一个阅读框位移，什么意思啊，教我如何是好，求！ 回信如下： e are currently processing your GenBank records KP168713, KP218513 and have come across an issue that requires your attention. Upon doing an alignment of your sequences, we noticed that there is a reading frame shift in both sequences near their 5' ends: KP168713 1 GGTCAACAAAATCATAAAGATATTGGTATACTTTATTTTATTTTTGGAATCT KP218513 1 GGTCAACAAAATCATAAAGATATTGGAACTTTATATTTTATTTTTGGGGCTT 197110089 1 GGTCAACAAA-TCATAAAGATATTGGAATTTTATATTTTATTTTTGGGATTT 586695063 2 GGTCAACAAA-TCATAAAGATATTGGAATTTTATATTTTATTTTTGGGATTT 575503652 25 TCAACAAA-CCACAAAGACATTGGAACTTTGTATTTCATCTTTGGGGCTT 261876073 1 AA-CCATAAAGATATTGGAACTTTATACTTTATTTTTGGGGCTT 261876033 1 AA-CCATAAAGATATTGGAACATTATATTTTATTTTTGGGGCTT 45774897 25 AA-TCATAAAGATATTGGAATATTATATTTTATTTTTGGAATCT 51922073 25 AA-TCACAAAGATATTGGAATGCTTTATTTTATTTTTGGAATTT ^^^ If the nucleotide sequences are incorrect, send us the corrected sequences, and we will replace the current sequences in your submission. If the sequences are correct, please let us know if you have any evidence that these frame shifted proteins are translated. |
回复此楼» 猜你喜欢
筑牢营养安全线：以精准检测，护健康基石
已经有0人回复
-
推荐一些20种氨基酸检测的实际应用案例
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有90人回复
-
不合理蛙科研实验中的趣事：实验器材的 “乌龙”
已经有0人回复
-
不合理蛙科研实验中的趣事：和实验材料的 “斗智斗勇”
已经有0人回复
-
蛋白质检测：精准分析，解锁生物分子的密码
已经有0人回复
-
不合理蛙科研实验之小鼠实验：严谨设计，解析生命机制的重要载体
已经有0人回复
-
不合理蛙科研实验之重金属检测：精准筛查，守护健康与环境的防线
已经有0人回复
-
不合理蛙科研实验之“蛙测重金属我背锅三千”
已经有0人回复
-
不合理蛙科研实验之“鼠逃三次我发三篇SCI”
已经有0人回复
zealoushebe
木虫 (初入文坛)
- 应助: 0 (幼儿园)
- 金币: 1927.1
- 红花: 1
- 帖子: 35
- 在线: 17.1小时
- 虫号: 3019447
- 注册: 2014-03-05
- 专业: 中药鉴定
2楼2017-06-20 09:48:14