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LRR exchange between homologues at the maize Rp1 locus results in a necrotic phenotype (138). Likewise, domain swaps between Mi-1.2 and its paralogue Mi-1.1 resulted in autoactivating incompatibilities between the LRR of Mi-1.2 and either the first 161 amino acids of the amino terminal domain (NT-1), or the next 751 residues encompassing the NB-ARC domain of Mi-1.1 (55). Autoactivating fusions were also obtained by swapping different, nonoverlapping, LRR sequences in Rx for the equivalent region of Gpa2 (116). These experiments suggest that the different domains interact with each other and that mispairing can result in spontaneous activation of the protein. |
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