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@木笔@木虫 (小有名气)
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[求助]
一段关于蛋白专业的英文翻译，烦请译成中文，多谢。
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The successful crystallization of TXS required co-crystallization with Mg21 and either 13-aza-13,14-dihydrocopalyl diphosphate (ACP) or 2-fluorogeranylgeranyl diphosphate (FGP) (molecular structures are shown in Supplementary Fig. 1). Although not active, this truncation variant is exceptionally stable and is the only form examined that yielded satisfactory crystals. Surprisingly, TXS contains three a-helical domains and harbours the folds of both class I and class II terpenoid cyclases (Fig. 2); this structure is representative of nearly all diterpene cyclases. The C-terminal domain (S553–V862) has the class I terpenoid synthase fold that was first observed in farnesyl diphosphate synthase23 and subsequently observed and designated the class I terpenoid synthase fold8,14 in monoterpene and sesquiterpene cyclases7–9. This fold is also observed in geranylgeranyl diphosphate synthase24, which generates the substrate for diterpene cyclases. The N-terminal domain of TXS (M107–I135 and S349–Q552) together with the ‘insertion’ domain25 (S136–Y348) comprise the double a-barrel class II terpenoid synthase fold that was first observed in the triterpene cyclase squalenehopene cyclase10 and later observed in oxidosqualene cyclase26. TXS shares no significant overall amino-acid sequence identity with these triterpene cyclases. |
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【答案】应助回帖
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@木笔@: 金币+30, 翻译EPI+1, ★★★很有帮助 2015-03-10 20:11:15
@木笔@: 金币+30, 翻译EPI+1, ★★★很有帮助 2015-03-10 20:11:15
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TXS的成功结晶需要与Mg21 和13-aza-13, ACP或FGP共结晶（分子结构见附图1）。 虽然没有活性， 这种截短的变异体异常稳定， 而且是唯一一种可以产生满意结晶的。令人惊讶的是， TXS含有3个螺旋结构域，而且含有I类和II类的 terpenoidcyclases 折叠(Fig. 2)。C 末端 (S553–V862) 含有I 类的terpenoid synthase折叠，首先在 farnesyl diphosphate synthase23中被发现，然后在monoterpene 和sesquiterpene cyclases7–9 中被发现和命名。这种折叠也在geranylgeranyl diphosphate synthase24 上发现, 并提供了 diterpene cyclases的底物。TXS 的N末端(M107–I135 and S349–Q552) 和 ‘插入’域25 (S136–Y348) 组成了双桶状的 II 类terpenoid synthase 折叠。 这种折叠首先在 triterpene cyclase squalenehopenecyclase10 上发现， 然后在oxidosqualene cyclase26上被发现. TXS和这些triterpene cyclases没有明显的氨基酸序列上的相似性。 |
2楼2015-03-10 11:35:57
