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1 Experiments designed to investigate the influence of the nitrite
concentration revealed the reaction to be second order with respect to this reagent.
2 Figure 3 shows the results obtained on working with different initial concentrations of each amino acid , first order being observed with respect to the amino acid concentration.
3 The concentrations of the species A, B, C, and D can be expressed as a function of the acidity and the total concentration of the amino acid in the working conditions.
4 The values of the microscopic constants can be estimated from KI and KII by assuming the microscopic constant K1 to be approximately equal to the acidity constant of an ester of the amino acid Ke.
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