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Enzybiotics - Antibiotic Enzymes As Drugs & Therapeutics - Wiley (2012)
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PREFACE vii CONTRIBUTORS ix 1 ENZYBIOTICS AND THEIR POTENTIAL APPLICATIONS IN MEDICINE 1 Jan Borysowski and Andrzej Górski 2 ADVANTAGES AND DISADVANTAGES IN THE USE OF ANTIBIOTICS OR PHAGES AS THERAPEUTIC AGENTS 27 Patricia Veiga-Crespo and Tomas G. Villa 3 ENZYBIOTICS AS SELECTIVE KILLERS OF TARGET BACTERIA 59 Juan C. Alonso and Marcelo E. Tolmasky 4 PHYLOGENY OF ENZYBIOTICS 75 Patricia Veiga-Crespo and Tomas G. Villa 5 BACTERIOPHAGE LYSINS: THE ULTIMATE ENZYBIOTIC 107 Vincent A. Fischetti 6 BACTERIOPHAGE HOLINS AND THEIR MEMBRANEDISRUPTING ACTIVITY 123 María Gasset 7 ANTI-STAPHYLOCOCCAL LYTIC ENZYMES 149 Jan Borysowski and Andrzej Górski v vi CONTENTS 8 MEMBRANE-TARGETED ENZYBIOTICS 173 María Gasset 9 DESIGN OF PHAGE COCKTAILS FOR THERAPY FROM A HOST RANGE POINT OF VIEW 199 Lawrence D. Goodridge 10 IDENTIFYING PHAGE LYTIC ENZYMES: PAST, PRESENT, AND FUTURE 219 Jonathan E. Schmitz, Raymond Schuch, and Vincent A. Fischetti 11 USE OF GENETICALLY MODIFIED PHAGES TO DELIVER SUICIDAL GENES TO TARGET BACTERIA 253 Lawrence D. Goodridge CONCLUDING REMARKS: THE FUTURE OF ENZYBIOTICS 269 Patricia Veiga-Crespo and Tomas G. Villa INDEX 279 PREFACE The writing of a preface on an old - yet - new subject, such as the one in this book, is always a diffi cult task. It is common knowledge that the discovery of penicillin, and subsequently the rest of the antibiotics, has probably been one of the most important scientifi c contributions to civilization. By saving millions of lives, antibiotics automatically increased the half - life of mankind, thus allowing scientists to give their best to society for 20 to 30 additional years. It is also common knowledge that resistance to antibiotics is a constant possibility and unfortunately something to be considered every time a new antibiotic goes on the market. Because of this, and because the discovery and design of new antibiotics becomes more and more diffi cult every year, society, through the work of several worldwide research groups, is looking into the use of what one of us (Dr. Vincent Fischetti) has termed “ enzybiotics ” (the result of blending the words “ enzymes ” and “ antibiotics ” ), for treating bacterial and fungal diseases, either alone or in combination with antibiotics. The book starts with four chapters in which the potential, advantages, and phylogeny of enzybiotics are reviewed. Then, the new ways of controlling infections by Gram - negative bacteria and an updated view of bacteriophage holins are presented. After a review of antistaphylococcal lytic enzymes, the book goes on to discuss membrane - targeted enzybiotics, as well as the design of phage cocktails for current therapy. Finally, the last two chapters deal respectively with the novel methods to identify new enzybiotics and the use of modifi ed phages to induce suicide in bacteria. All in all, the contributors are all active researchers, involved in the topic of enzybiotics. It is hoped that the joining of different points of view, such as those refl ected in this book, will help to clarify the vii |
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