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肖丽凤铜虫 (小有名气)
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Sample application Apply the prescribed volume of the solutions at a suitable distance from the lower edge and from the sides of the plate and on a line parallel to the lower edge; allow an interval of at least 10mm (5mm om high-performance plates) between the centres of circular spots and 5mm (2mm on high-performance plates) between the edges of bands. Apply the solutions in sufficiently small portions to obtain circular spots 2-5 mm in diameter(1-2mm on high-performance plates) or bands 10-20mm(5-10mm on high-performance plates)by 1-2mm. In a monograph, where both normal and high-performance plates may be used, the working cinditions for high-performance plates are given in the brackets after those for normal plates. Vertical development. Line the walls of the chromatographic tank with filter paper. Pour into the chromatographic tank a sufficient quantity of the molile phase for the size of the tank to give after impregnation of the filter paper a layer of appropriate depth related to the dimension of the plate to be used. For saturation of the chromatographic tank, replace the lid and allow to stand at 20-25℃ for 1h.Unless otherwise indicated in the monograph,the chromatographic separation is performed in a saturated tank. Apply the prescribed volume of solutions as described above.When the solvent has evaporated from the applied solutions, place the plate in the chromatographic tank, ensuring that the plate is as vertical as possible and that the spots or bands are above the surface of the mobile phase. Close the chromatographic tank, maintain it at 20-25℃ and protect from sunlight. Remove the plate when the mobile phase has moved over the prescribed distance, measured between the points of application and the solvent front. Dry the plate and visualize the chromatograms as prescribed. For two-dimensional chromatography, dry the plates after the first development and carry out a second development in a direction perpendicular to that of the first development. |
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chengcj.2008
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2楼2008-06-12 18:41:58
肖丽凤
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3楼2008-06-13 07:57:07
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