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huojinlong8610: 金币+100, 翻译EPI+1 2015-02-11 15:28:27
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PPARδ is a nuclear hormone receptor that governs a variety of biological processes, including fatty acid metabolism. For studying the regulation and function of PPARD in pigs, PPARδ cDNA including the coding sequence was obtained by RT-PCR with the RNA isolated from pigs and submitted to GenBank with the access number. Bioinformatics analysis showed that the PPARδ gene encode a protein of 462 amino acids with a predicted molecular weight of 49.74 kD and the isoelectric point of 7.53. Protein structure analysis showed that PPARδ protein contains two conserved domains and a leucine-rich nuclear export signal without transmembrane regions and signal sequence． Hydrophobicity analysis indicate that the N-terminus and C-terminus are hydrophilic. The probability of secreting into periplasmic is 69.6%. Phylogenetic results indicated that the pig has the closest relationship with cattle and dog. Two different nucleotides (A or G) at 95 nucleotide in the coding region of the PPARδ gene and three genotypes were found: AA, AB, BB. PPARδ mRNA expression in 14 tissues was tested by qPCR and the result showed that the expression was high in liver, spleen, ear and small intestine. This study provides experimental basis for further study on the expression and regulation of PPARδ gene. |
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