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克拉克肯特至尊木虫 (著名写手)
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[求助]
求组spa和hla 基因蓝白筛选问题
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如图，不太明白spa和hla 基因蓝白筛选问题 Schematic representation of the X-GAL agar plate test, which can be used in QSI screening efforts of either individual compounds or more complex natural product extracts. QSIs are identified by presence of both a white ring in the hla::lacZ plate and blue ring on the spa::lacZ plate. (A) hla::lacZ plate: following incubation of the plate containing the hla reporter, a white clearing surrounding the hole punch containing the QSI candidate indicates reduced expression of hla (α-toxin). (B) spa::lacZ plate: following incubation of the plate containing the spa reporter, a blue zone surrounding the hole punch containing the QSI candidate indicates increased expression of spa (Protein A).  fmicb-05-00706-g003.jpg |
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