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专业: 森林生物学

[求助] methylammonium/ ammmonium permease 怎么翻译

The ammoniummethylammonium transport (Amt) proteins of
enteric bacteria and their homologues, the methylammonium
ammonium permeases ofSaccharomyces cerevisiae, are required
for fast growth at very low concentrations of the uncharged
species NH3. For example, they are essential at low ammonium
(NH4
NH3) concentrations under acidic conditions. Based on
growth studies in batch culture, the Amt protein ofSalmonella
typhimurium(AmtB) cannot concentrate either NH3or NH4

and
this organism appears to have no means of doing so. We now show
thatS. typhimuriumreleases ammonium into the medium when
grown on the alternative nitrogen source arginine and that outward diffusion of ammonium is enhanced by the activity of AmtB.
The latter result indicates that AmtB acts bidirectionally. We also
confirm a prediction that the AmtB protein would be required at pH
7.0 in ammonium-limited continuous culture, i.e., when the concentration of NH3is <50 nM. Together with our previous studies,
current results are in accord with the view that Amt and methylammoniumammonium permease proteins increase the rate of
diffusion of the uncharged species NH3 across the cytoplasmic
membrane. These proteins are examples of protein facilitators
for a gas.

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