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lvmuzhi

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[求助] 16s rDNA比对用的EZ很不正常,换到RDP,无法显示出相似度。烦请指教!

不知道大家最近有没发现EZ的问题,最近比对结果不对劲,不正常。
换到RDP网站 https://rdp.cme.msu.edu/index.jsp,比对结果不显示相似度,查找原因:
After uploading my sequences and utilizing the SEQMATCH function my sequences produce a S_ab score but the similarity score says "not calculated". How can I get a similarity score for these DNA sequences?

Pairwise similarity values are only calculated by SeqMatch IF the sequences have been aligned by RDP. Any sequences DIRECTLY selected from RDP through Hierarchy Browser or from myRDP aligned sequences will have pairwise similarity values calculated and displayed by Sequence Match. The query sequences through copy&paste or file uploading to Sequence Match will not display the similarity values.
谁知道因为什么吗?看不太懂。
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