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chunyan159金虫 (小有名气)
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[求助]
求翻译 测序组装的
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De novo assembly. First, we generated a 17-mer depth distribution of shortinsert paired-end reads using Meryl50 and applied GCE51 to estimate the genome sizes of individual G. soja accessions. Reads were preprocessed by ALLPATHS-LG52 error correction module to remove base calling errors. We also used ErrorCorrection in SOAPdenovo11 package to connect 180-bp library pair end reads and to generate longer sequences for assembly. Reads of 180-bp and 500-bp library were used for contig building, and all pair-end reads libraries were used to provide links for scaffold construction. GapCloser (v1.12) from SOAPdenovo11 package was used for gap filling within assembled scaffolds using all pair-end reads. Finally, scaffold sequences, which can be aligned to bacterial genomes with identity ≥95% and e-value ≤1e-5, were filtered。 Genomic alignment and short read mapping |
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