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Saccharomyces cerevisiae 的strain
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我们平时说的Saccharomyces cerevisiae是一个笼统的称呼么？还是说Saccharomyces cerevisiae是指wild type？Saccharomyces cerevisiae A364A是其Strain中的wild type么？Saccharomyces cerevisiae 有多少strain呢？哪里可以查到相关信息。 谢谢了 |
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3楼2008-05-26 21:54:58
2楼2008-05-26 10:01:15
有关于详细介绍Saccharomyces cerevisiae的相关书籍么？
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网上只有对其基因变异型的详细介绍。 部分PDF copy 下来如下 SACCHAROMYCES CEREVISIAE – OVERVIEW EXAMPLES ade5 cdc28 CUP1 SPC105 act1-606 his2-1 Ade5p Cdc28p Cup1p Spc105p Arg– (cf. wild type Arg+) SWI SWI1 SWI3 SWI5 etc. YKL025C (a) ade6::URA4 (b) ade6-Δ1 (c) ade6::URA4 SUP4 SUF1 sup35 suf11 SUF1 or suf1 (a) ssn1 (b) srn1 (c) suh1 SUP4-o SUP4-a sup4-o-1 GENES (LOCI) Gene symbols comprise three italic lowercase letters, and an Arabic number (full gene names are not controlled by the nomenclature system). Symbols are styled according to the phenotype of the identifying mutation or for the function of the wild-type gene (see ‘Genes’ and ‘Alleles’ for more details): lowercase italic for recessive, uppercase italic for dominant. ALLELES Allele designations consist of the gene symbol, a hyphen and an italic Arabic number. PROTEINS Proteins are referred to by the relevant gene symbol, non-italic, initial letter uppercase and with the suffix ‘p’ (to avoid confusion with the phenotype, see below). If unambiguous, the suffix can be omitted e.g. ‘the Ade5 protein’. PHENOTYPES Phenotypes are designated by a non-italic three-letter abbreviation corresponding to the gene symbol, initial letter uppercase. Wild-type or mutant status is indicated by a superscript plus or minus sign, respectively, e.g. a strain requiring arginine. GENES As mentioned above, for genes defined by mutation, upper- and lowercase designations are used for dominant and recessive alleles, respectively. However, because a given allele can be dominant in one cross and recessive in another, this can lead to some difficulty. On the genetic and physical maps, the convention is to use the mapped allele to decide which form of the name is used. Genes with related properties are usually given the same three-letter name and different numbers, e.g. there are multiple genes that have functions in mating-type switching. Open reading frame (ORF) designations are not gene names but ‘location holders’ on the genetic map until a gene name is assigned. ORF names are always three non-italic uppercase letters, a number and a letter: Y (for yeast unknown sequence); A, B to P (for chromosome I, II through XVI); R or L (for right or left arm); a number corresponding to the order of the ORF (counting from the centromere), and W or C to designate Watson or Crick strand (the Watson strand is 5→3 left telomere to right telomere), e.g. the 25th ORF on the left arm of chromosome XI. Mitochondrial mutations should, in general, be designated following the rules outlined above, but well-known symbols, such as +, –, + and –, have been retained. Detailed designations have been published for mitochondrial mutants1 and killer strains2. ALLELES Alleles created by recombinant DNA technology should be named by use of the symbol for the gene that is altered, followed by a symbol to indicate the nature of the alteration: disruption (:  ; deletion (-Δ); replacement (Δ:.e.g. (a) Disruption of the ade6 gene by integration by the functional URA4 gene. (b) Deletion number 1 of the ade6 gene. (c) Replacement of ade6 by the URA4 gene. Dominant and recessive suppressors are designated by three upper- or lowercase letters, respectively, and a locus number. Frameshift suppressors are normally designated in upper- or lowercase. Metabolic suppressors can be designated in various ways, e.g. (a) a suppressor of snf1; (b) a suppressor of rna1-1; (c) a suppressor of his2-1. Ochre and amber suppressors are sometimes distinguished by a bold-face suffix -o or -a. Intragenic mutations that inactivate suppressor function are designated by the same rules as other mutant alleles. |
4楼2008-05-26 21:58:31