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[求助]
求职翻译摘要，快毕不了业了
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摘 要：探讨病毒Ad35-SF感染T细胞后表达SIRPα-Fc（SF）融合基因蛋白对T细胞功能的影响。以包装含有SF基因的腺病毒Ad35-SF感染Jurkat细胞48h，Western blotting检测结果显示SF蛋白大小约为48kD,与理论值吻合，ELISA检测表明该蛋白在细胞上清中的表达量为19.1μg/ml。Ad35-SF（MOI=1）感染Jurkat细胞8h后，CD47阳性比例从97.06％下降到15.32％；当Ad35-SF感染复数增加至5,10时，CD47阳性比率进一步降低至2.94%和1.73%。相对参照组，Ad35-SF感染Jurkat细胞48h后其增殖效果提高了29％，对肝癌细胞株Hep 3B的杀伤作用提升25.6％。该研究表明构建的病毒Ad35-SF能有效感染Jurkat细胞并分泌SF蛋白，从而封闭其表面的CD47。感染后的Jurkat细胞会继续分泌SF蛋白并封闭其它未被感染的Jurkat细胞，从而显著提高Jurkat细胞的增殖能力和对肝癌细胞的杀伤作用。 高手们帮帮忙，确实英语差，马上要毕业了，还没投稿，快帮帮忙吧，谢谢 |
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cwq921: 金币+50, ★★★★★最佳答案, 帮实验室的，谢谢啦！ 2014-12-15 12:45:43
cwq921: 金币+50, ★★★★★最佳答案, 帮实验室的，谢谢啦！ 2014-12-15 12:45:43
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Abstract: To investigate the effect of expression of SIRPα-Fc (SF), a fusion protein encoded by adenovirus Ad35-SF, on T cell function, Jurkat cells were infected with adenovirus Ad35-SF for 48 h. Western blotting analysis showed that the expressed fusion protein SF was about 48 kD, which was consistent with the calculated value. ELISA assay showed that the concentration of the fusion protein in the culture medium was 19.1 μg/ml. CD47-positive cells decreased dramatically from 97.06% to 15.32% when Jurkat cells were infected with Ad35-SF [multiplicity of infection (MOI)=1] for 8h, and further decreased to 2.94% and 1.73%, respectively, if MOI was increased to 5 and 10. The proliferation of Jurkat cells 48 h after Ad35-SF infection increased by 29% and increased the killing effect of of the infected Jrukat cells to hepatocellular carcinoma cell line Hep3B by 25.6%, as compared with the controls. The results of this study showed that the adenovirus Ad35-SF we made can efficiently infect Jurkat cells and express functional secretory protein SF, thereby blocking the cell surface marker CD47. In addition, the infected Jurkat cells will continue to secrete SF protein and block other uninfected Jurkat cells, which can significantly improve the proliferation of Jurkat cells and their cytotoxicity to hepatocellular carcinoma cells. 注： 1、没有完全按照你的中文一对一翻译（尽可能按你的原话），而是根据英文表达习惯按照你要表达的内容做了适当调整。 2、你的中文有许多地方需要调整，如第一句和第二句之间有一种目的关系，故而不宜用句号。倒是后面一连串的逗号需要重新断句。再如”细胞上清中的表达量为19.1μg/ml“，我明白你在说什么，但严格说来所谓细胞上清应该是指离心后的培养基（相对于条件培养基conditional medium），故而我在翻译时用了culture medium而非cell supernatants；另外，你确定蛋白浓度是19.1 μg/ml而不是19.1 ng/ml吗？ 希望有帮助，尽早毕业！ |
2楼2014-12-15 12:09:56
