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海晏河清lee

木虫 (小有名气)

[求助] 一篇JACS文章摘要求翻译

本人基本看明白了,但是书面翻译的时候总是说不成流利的中文,求大神帮翻译成漂亮的中文,金币可以追加!

题目Finding the Switch: Turning a Baeyer–Villiger Monooxygenase into a NADPH Oxidase

By a targeted enzyme engineering approach, we were able to create an efficient NADPH oxidase from a monooxygenase. Intriguingly, replacement of only one specific single amino acid was sufficient for such a monooxygenase-to-oxidase switch—a complete transition in enzyme activity. Pre-steady-state kinetic analysis and elucidation of the crystal structure of the C65D PAMO mutant revealed that the mutation introduces small changes near the flavin cofactor, resulting in a rapid decay of the peroxyflavin intermediate. The engineered biocatalyst was shown to be a thermostable, solvent tolerant, and effective cofactor-regenerating biocatalyst. Therefore, it represents a valuable new biocatalytic tool.
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沉沦不了情

木虫 (正式写手)

JIN CHONG

JACS是美国化学会志?文章句子不难,生词好多
机遇总是垂青于有准备的人。。。
2楼2014-12-13 22:54:01
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