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★★★★★★2014书籍超级帖九五五《神经干细胞分析》2014最新书籍
书名Neural Stem Cell Assays
作者Navjot Kaur
Mohan C. Vemuri
出版社wiley
页数312
ISBN 978-1-118-30825-7 (cloth)
Table of Contents
Contributors xv
Preface xix
1 Neural Differentiation of Pluripotent Stem Cells 1
Mohan C. Vemuri
1.1 Introduction 1
1.2 NSC Derivation from Rosette Formation of Embryoid Bodies 1
1.3 Rosette Free NSC in a Monolayer Culture 2
1.4 Pathways Involved in Neural Tube and Neural Crest Lineages 2
1.5 Differentiation and Gene Expression in Human Brain Development 3
1.5.1 Differentiation to Forebrain Neurons from Pluripotent Stem Cells 3
1.5.2 Differentiation to Midbrain Dopaminergic Neurons from Pluripotent
Stem Cells 4
1.5.3 Differentiation to Motor Neurons from Human Pluripotent Stem Cells 5
1.6 Conclusions 6
References 6
2 The History and Design of Assays for the Identification and Characterization of Neural
Stem Cells 9
Sharon A. Louis and Brent A. Reynolds
2.1 Introduction 9
2.1.1 Stem Cells 9
2.1.2 Stem Cells in the Central Nervous System 10
2.1.3 The Neurosphere Assay 11
2.2 Materials 12
2.2.1 General Equipment 12
2.2.2 Tissue Culture Equipment (Neurosphere Culture) 12
2.2.3 Media, Supplements and Associated Reagents for Neurosphere Cultures 13
2.2.4 Media, Supplements, and Tissue Culture Equipment for Neural Colony
Forming Cell Assay 13
2.3 Methods 14
2.3.1 Neurosphere Cultures from Primary Embryonic or Adult CNS Tissues 14
2.3.2 Neural Colony Forming Cell (NCFC) Assay for Enumerating Neural
Stem Cells and Neural Progenitor Cells 18
2.3.3 Categorizing NCFC Colonies and Scoring NCFC Colonies 20
2.3.4 Application of the NCFC Assay for Estimation of NSCs or Neural
Progenitors 21
2.4 Notes 21
Acknowledgments 23
References 23
3 Culture of Human Neural Stem Cells 25
Soojung Shin, Yiping Yan and Mohan C Vemuri
3.1 Introduction 25
3.2 Materials 25
3.2.1 Reagents List 25
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