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bluedance

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[交流] DE3作为宿主菌的特性?

有谁了解DE3作为基因宿主菌在进行载体转入时的特性及注意事项
急。。。。
谢了
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wjswj

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小木虫领金币专业户

Promega 得BL21 cell, discription 这样说:Bl21(DE3)pLysS can be used with
protein expression vectors that are under the control of the T7 promoter, such
as pET vectorers. This strain is lysogenic for lambda-DE3(4), which contains
the T7 bacteriophage gene1, encoding T7 RNA polymerase under the control of
the lac UV5 promoter. BL21(DE3) pLysS also contains the pLysS palsmid, which
carries the gene encoding T7 lysozyme. T7 lysozyme lowers the background
expression level of target genes under the control of the T7 promoter but does
not inerfere with the level of expression achieved following induction with
IPTG.
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2楼2008-05-23 12:01:11
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