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3.7. Cell morphology adhered on PLGA/PHBV scaffold
As shown in Fig. 7, the cell morphology of hMSCs grown on three kinds of scaffolds showed some differences at 12 h. The cells had grown into the surface by forming filopodia between some apertures. Most of the cells seemed to prefer to adhere onto the surface of PHBV microspheres than onto the morphology of PLGA matrix made by NaCl particles. This point can be approved by graphs C, D, E, and F in Fig. 7. Most of the visible cells were lain on or between PHBV microspheres.
5. Conclusion
In this study, we set up a new model which was composed of PLGA and PHBV for bone repair. After incorporating PHBV microspheres into PLGA scaffold, morphology, pore size distribution,porosity, compressive strength and in vitro experiments were employed to evaluate it. The PLGA/PHBV scaffold has good pore interconnectivity and macropores which is suitable for cells to grow in. Besides, by mixing the PHBV microspheres into PLGA matrix, the compressive strength of the scaffold was improved obviously while the morphology was not destroyed. The in vitro evaluation showed that the new model can facilitate the cells to proliferate well. In a word, the PLGA/PHBV model is worth further studying for a bone repair application.

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