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3.7. Cell morphology adhered on PLGA/PHBV scaffold As shown in Fig. 7, the cell morphology of hMSCs grown on three kinds of scaffolds showed some differences at 12 h. The cells had grown into the surface by forming filopodia between some apertures. Most of the cells seemed to prefer to adhere onto the surface of PHBV microspheres than onto the morphology of PLGA matrix made by NaCl particles. This point can be approved by graphs C, D, E, and F in Fig. 7. Most of the visible cells were lain on or between PHBV microspheres. 5. Conclusion In this study, we set up a new model which was composed of PLGA and PHBV for bone repair. After incorporating PHBV microspheres into PLGA scaffold, morphology, pore size distribution,porosity, compressive strength and in vitro experiments were employed to evaluate it. The PLGA/PHBV scaffold has good pore interconnectivity and macropores which is suitable for cells to grow in. Besides, by mixing the PHBV microspheres into PLGA matrix, the compressive strength of the scaffold was improved obviously while the morphology was not destroyed. The in vitro evaluation showed that the new model can facilitate the cells to proliferate well. In a word, the PLGA/PHBV model is worth further studying for a bone repair application. |
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LifePursuit
木虫之王 (文学泰斗)
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3.7.黏附于PLGA/PHBV支架材料的细胞形貌 如图7所示，在三种支架材料上生长的hMSCs细胞形貌在12小时时显示出某些不同。hMSCs细胞通过在某些孔隙间形成丝状伪足而生长进入表面。大多数的细胞看起来更倾向于黏附在PHBV微球的表面而不是由NaCl粒子形成的PLGA集体的表面。图7中C、D、 E及F图可以证明这一点。大多数可见的hMSCs细胞位于PHBV微球上，或者在PHBV微球之间。 5. 结论 在本研究中，我们建立起一个由PLGA和PHBV构筑的新模型从而实现骨修复。当PHBV微球被引入PLGA支架材料后，我们通过形貌、孔径分布、孔隙率、压缩强度和体外实验等参数来评价这一新模型。PLGA/PHBV支架材料具有很好的孔隙连接性，同时富含适合细胞生长的大孔。此外，通过将PHBV微球引入PLGA基体中，支架材料的压缩强度显著提升，而形貌则保持不变。体外评价显示这一新模型可以很好地促进细胞增殖。总而言之，PLGA/PHBV模型作为骨修复材料值得进一步研究。 |
2楼2014-12-14 19:35:20
