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3.8. Viability of cells cultured on PLGA/PHBV scaffolds Viability of cells cultured on PLGA/PHBV scaffolds was evaluated by means of kinetic examinations and observation of hMSCs, which was quantified by MTT and Live/Dead assay respectively. As shown in Fig. 8, cell viability of the cells on each group of scaffolds increased gradually when the culture days went longer. However,there was no significant differentiation between the cell viability of all groups on day1, day 3 and day 7. The result indicated that the adding of PHBV microspheres had no significant influence on the cell viability. The viability of hMSCs on the scaffolds was generally visualized using fluorescent Live/Dead assay. Fig. 9 indicated the cells status at day 7 and 14 after being seeded on three kinds of scaffold. On day 7, few dead cells (red spots) can be observed, which indicated that at the first few days there were several dead cells with damaged membranes [35]. With the increase of incubation time, more and more green spots can be observed which indicated that the cell proliferated well on the scaffolds. Besides, some graphs showed that the green light formed several circles which indicated that the cells were attached on the microspheres, which was coincident with the results of SEM micrographs. |
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2楼2014-12-08 08:58:23
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雪嫣2: 金币+50, 翻译EPI+1, ★★★★★最佳答案, 非常感谢你的回复,我很满意，很棒！谢谢你哈！ 2014-12-08 16:58:28
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培养在PLGA/PHBV支架上的细胞的活性测试 培养在PLGA/PHBV支架上的细胞的活性借助于动力学检查（MTT测试）和对hMSCs的观察（活/死法）进行评估。如图8所示，每组支架上的细胞的活性随着培养天数的增长而增加。然而，所有组的细胞活性在第1、3、7天没有明显差异。这一结果表明，PHBV微球的加入对细胞活性没有明显影响。hMSCs在支架上的活性通常通过荧光活/死法进行可视化检测。图9显示种在3中支架上的细胞在第7天和第14天的状态。在第7天时，能观察到少许死细胞（红色的点），这表明在最初的几天有少数细胞随细胞膜破坏而死亡。随着孵育时间的延长，可观察到越来越多的绿色的点，表明细胞能在支架上很好地增殖。此外，一些图中出现了绿光形成的圆圈，表明细胞粘是附在微球上的，这与SEM现实的结果一致。 |
3楼2014-12-08 16:49:39